中国水稻科学 ›› 2016, Vol. 30 ›› Issue (5): 479-486.DOI: 10.16819/j.1001-7216.2016.6037

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水稻矮秆多分蘖突变体mz3的遗传分析和基因定位

苏晓妹1, 方宇星1, 刘钰龙1, 刘峰1, 张所兵2, 张云辉2,*(), 鲍依群1,*()   

  1. 1南京农业大学 生命科学学院, 南京 210095
    2江苏省农业科学院 粮食作物研究所/江苏省农业种质资源保护与利用平台,南京 210014
  • 收稿日期:2016-03-04 修回日期:2016-05-02 出版日期:2016-09-10 发布日期:2016-09-10
  • 通讯作者: 张云辉,鲍依群
  • 基金资助:
    国家自然科学基金资助项目(31401036);江苏省自然科学基金资助项目(BK20130706);江苏省农业科技自主创新资金资助项目[(CX(14)5005)];江苏省农业科学院基本科研业务专项[(ZX(15)4015)]

Genetic Analysis and Gene Mapping of a Dwarf and High-tillering Mutant mz3 in Rice (Oryza sativa L.)

Xiao-mei SU1, Yu-xing FANG1, Yu-long LIU1, Feng LIU1, Suo-bing ZHANG2, Yun-hui ZHANG2,*(), Yi-qun BAO1,*()   

  1. 1College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
    2Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm/Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Received:2016-03-04 Revised:2016-05-02 Online:2016-09-10 Published:2016-09-10
  • Contact: Yun-hui ZHANG, Yi-qun BAO

摘要:

通过EMS诱变粳稻品种中花11获得一个稳定遗传的矮秆多分蘖突变体mz3。遗传分析表明该突变性状受一对隐性基因控制,并利用mz3与籼稻品种南京11杂交建立的F2群体,将该基因定位在水稻第6染色体长臂上的SSR标记RM19353与RM510之间约747 kb范围内。由于该区间包含控制水稻株高和分蘖的D3基因,结合表型分析,推测突变基因与D3可能为一对等位基因。设计7对引物分别对中花11与突变体mz3的基因进行测序,结果显示,与中花11相比,D3基因在mz3中第636位核苷酸由G突变为A,使得编码色氨酸的密码子TGG突变为终止密码子TGA,导致翻译提前终止。进一步对定位群体中10个隐性极端个体测序,结果显示所有极端个体都带有该突变位点。亚细胞定位结果表明,突变体编码的D3蛋白与野生型一样定位在细胞核中,荧光双分子互补试验结果表明,突变体D3蛋白不能与D14蛋白发生互作,推测突变体编码的D3截短蛋白缺少了与D14互作的氨基酸序列,从而阻碍了独脚金内酯信号传递。因此,mz3表型很可能由D3基因突变引起。

关键词: 水稻, 矮秆多分蘖, D3, 基因定位

Abstract:

A dwarf and high-tillering mutant, temporarily termed as mz3 was obtained from Zhonghua 11, a japonica rice variety by EMS mutagenesis. Genetic analysis indicated that the mutant phenotype was controlled by a recessive gene. An F2 population was developed by crossing mz3 with Nanjing 11, an indica rice variety for mapping of the mutant gene, which was ultimately restricted within a physical distance of about 747 kb between two SSR markers RM19353 and RM510 on the long arm of rice chromosome 6. Gene prediction revealed that D3, a gene conferring rice plant height and tiller number was located in this region. Sequence analysis identified a single G to A nuclear acid substitution at the position 636 from the ATG start codon, forming a premature translational termination codon. Sequencing of 10 randomly selected recessive plants from mz3/Nanjing 11 mapping population confirmed the same mutation site carried by the 10 individuals. Subcelluar location indicated that D3 protein of the mutant was located in the nuclei as wild type. BiFC analysis showed that D3 protein of the mutant could not interact with D14 protein for lacking of essential amino acids that bind with D14, blocking signal transduction of strigolactones. So we speculated that the mutant phenotype of mz3 was probably caused by mutation in D3 gene.

Key words: rice, dwarf and high-tillering, D3, gene mapping

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